Natural resources and breeding of edible fungi; biology of the reproduction and its impact on the population structure; phylogeny and evolution; species concept and fungal taxonomy.
We have studied in details the different types of life cycles existing in Agaricus bisporus (the button mushroom) which remains today our main model. I am interested by natural biological processes occurring in situ (role played by the different reproductive modes and by the spores; recombination etc...). Using molecular genetics, we analyse wild populations and events occurring in given situations simulated in experimental culture. Such investigations have already offered new knowledge and methods useful for mushroom breeding or for the management of natural resources. These biological processes and certain potentially adaptative traits are also investigated at the genus level. I am interested by the species concept through different approaches (biological, phylogenetical, and ecological). Current research includes the following:
- biology and genetic of the reproduction in basidiomycete fungi
- relative roles played by different processes of outcrossing in artificial or wild populations
- variability/genetics of traits as color, volatile components, and fruiting temperature of sporocarps
- evaluation and conservation of the germ plasm of Agaricus species
- taxonomy and phylogenetic reconstruction of genus Agaricus
Genetics and biology (mainly of the reproduction)
Kamzolkina OV, Volkova VN, Kozlova MV, Pancheva EV, Dyakov YT, Callac P. 2006. Karyological evidence for meiosis in the three different types of life cycles existing in Agaricus bisporus. Mycologia 98:763-770.
Callac P, Spataro C, Caille A, Imbernon M. 2006. Evidence for outcrossing via the Buller phenomenon in a substrate simultaneously inoculated with spores and mycelium of Agaricus bisporus. Appl Environ Microb 72:2366-2372.
Callac P., Hocquart S., Imbernon M., Desmerger C., Olivier J. M., 1998. Bsn-t allele from French field strains of Agaricus bisporus. Appl Environ Microb 64: 2105-2110.
Callac P., Moquet F., Imbernon M., Ramos Guedes-Lafargue M., Mamoun M., Olivier J. M., 1998. Evidence for PPC1, a determinant of the pileipellis color of Agaricus bisporus fruitbodies. Fungal Genet Biol 23: 181-188.
Callac P., Desmerger C., Kerrigan R. W., Imbernon M., 1997. Conservation of genetic linkage with map expansion in distantly related crosses of Agaricus bisporus. FEMS Microbiol Lett 146: 235-240.
Imbernon M., Callac P., Gasqui P., Kerrigan R. W. et Velcko Jr A. J., 1996. BSN, the primary determinant of basidial spore number and reproductive mode in Agaricus bisporus, maps to chromosome I. Mycologia 88: 749-761
Kerrigan R. W., Imbernon M., Callac P., Billette C., Olivier J. M. 1994. The heterothallic life cycle of Agaricus bisporus var. burnettii, and the inheritance of its tetrasporic trait. Exp. Mycol. 18: 193-210.
Phylogeny, taxonomy, and genetic resources
Kerrigan RW, Callac P, Guinberteau J, Challen MP, Parra LA. 2006 [‘‘2005’’]. Agaricus section Xanthodermatei: a phylogenetic reconstruction with commentary on taxa. Mycologia 97:1292–1315.
Callac P., Guinberteau J., 2005. Morphological and molecular characterization of two novel species of Agaricus section Xanthodermatei.. Mycologia.97: 416-424.
Challen M. P., Kerrigan R. W., Callac P., 2003. A phylogenetic reconstruction and emendation of Agaricus section Duploannulatae, Mycologia 95: 61-71.
Callac P., Jacobe de Haut I., Imbernon M., Guinberteau J. Theochari I., 2003. A novel homothallic variety of Agaricus bisporus comprises rare tetrasporic isolates from Europe, Mycologia 95: 222-231.
Xu J., Desmerger C., Callac P., 2002. Fine-scale genetic analyses reveal unexpected spatial-temporal heterogeneity in two natural populations of the commercial mushroom Agaricus bisporus. Microbiol-SGM 148: 1253-1262
Kerrigan R. W., Callac P., Xu J. Noble R., 1999. Population and phylogenetic structure within the Agaricus subfloccosus complex. Mycol Res 103: 1515-1523.
Xu J., Kerrigan R. W., Sonnenberg A., Callac P., Horgen P. A., Anderson J.B., 1998. Mitochondrial DNA variation in natural populations of the mushroom Agaricus bisporus. Mol Ecol 7: 19-33.
Callac P., Billette C., Imbernon M., Kerrigan R. W., 1993. Morphological, genetic, and interfertility analyses reveal a novel, tetrasporic variety of Agaricus bisporus from the Sonoran Desert of California. Mycologia: 85: 335-351.