Cendoya E., Pinson-Gadais L., Farnochi M.C., Ramireza, M.L., Chéreau S., Marcheguay G., Ducos C., Barreau C., Richard-Forget F. (2017). Abiotic conditions leading to FUM gene expression and fumonisin accumulation by Fusarium proliferatum strains grown on a wheat-based substrate. International Journal of Food Microbiology 253: 12-19.
Fusarium proliferatum produces fumonisins B not only on maize but also on diverse crops including wheat. Using
a wheat-based medium, the effects of abiotic factors, temperature and water activity (aw), on growth, fumonisin
biosynthesis, and expression of FUM genes were compared for three F. proliferatum strains isolated from durum
wheat in Argentina. Although all isolates showed similar profiles of growth, the fumonisin production profiles
were slightly different. Regarding FUM gene transcriptional control, both FUM8 and FUM19 expression showed
similar behavior in all tested conditions. For both genes, expression at 25 °C correlated with fumonisin
production, regardless of the aw conditions. However, at 15 °C, these two genes were as highly expressed as at
25 °C although the amounts of toxin were very weak, suggesting that the kinetics of fumonisin production was
slowed at 15 °C. This study provides useful baseline data on conditions representing a low or a high risk for
contamination of wheat kernels with fumonisins.