Martinez Tuppia C., Atanasova-Penichon V. Chéreau S., Ferrer N., Marchegay G., Savoie J.M., Richard-Forget F. (2017). Yeast and bacteria from ensiled high moisture maize grains as potential mitigation agents of fumonisin B1. Journal of Science of Food and Agriculture 97: 2443–2452. DOI 10.1002/jsfa.8058
BACKGROUND: Fumonisin B1 (FB1) is a mycotoxin produced by several Fusarium species and is a very common contaminant
of maize-based food and feed throughout the world. The selection and use of FB1-degrading microorganisms appears as a
promising alternative to cope with the problem of toxicity towards humans and livestock. High moisture maize grain silage,
which is based on naturalmaize fermentation, could be an interesting reservoir of suchmicroorganisms.
RESULTS: Using an in vitro simulated silage model with FB1 naturally contaminated grains, we demonstrated a significant raw
decrease in FB1 during ensiling process ascribed to biodegradation mechanisms. A panel of 98 bacteria and yeastswere isolated
fromthismatrix and selected for their ability to use FB1 as the sole source of C andN. For nine of them, the ability to degrade FB1
in vitro was evidenced. Notably, two bacteria identified as Lactobacillus sp. were highlighted for their efficient FB1-degrading
capacity and production of hydrolysed FB1 as intermediate degradationmetabolite.
CONCLUSION: Fermentation of high moisture maize grain contaminated with FB1 leads to a significant reduction of the toxin
and allows the isolation of FB1-degrading microorganisms that could further be used as FB1 decontaminating agents.
Keywords: biodegradation; mycotoxin; fumonisin B1; hydrolysed FB1; silage