Quantitative analysis of gene expression

RNA analysis and real time PCR

Contacts : Jean-Christophe Helbling: jean-christophe.helbling@inrae.fr, Agnès Aubert: agnes.aubert@inrae.fr 

PCR1

Applications

  • Total RNA extraction
  • Absorbance based quantification of RNA on micro-volumes
  • Quality control of total RNA
  • cDNA synthesis
  • Relative quantification of mRNA expression by real-time PCR (qPCR) with SYBR I or TaqMan
PCR2

Equipments

  • TissueLyser II Qiagen (tissue disruption)
  • NanoDrop One (nucleic acid quantification)
  • Bioanalyzer 2100 Agilent (nucleic acid quality control)
  • Automated pipeting system Eppendorf epMotion 5070 (set-up of qPCR multiwell plates)
  • LightCycler 480 Roche (qPCR in 96 and 384 well plate formats)
  • Thermoclyclers for classic PCR and cDNA synthesis (96 well plates and tubs)
PCR3
PCR4

Modification date : 14 August 2023 | Publication date : 20 November 2013 | Redactor : Catherine