Facility for mice reproduction and génotypage

Facility for mice reproduction and génotypage

Facility for mice reproduction and génotypage                     

Official: Jean-Christophe Helbling: jean-christophe.helbling@inrae.fr

Technician: Celine Ducroix-Crepy: celine.ducroix-crepy@inrae.fr

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Goal: Reproduction and selection by genotypage

Methods

Fast ADN extraction from mice tail (5mm), simplex andmultiplex PCR, electrophoresis (agarose)

Home made « Hot Start Taq Pol » enzyme

11 different PCR to identify 10 lines and postnatal sex determination

-k.o. Cbg                       -Cx3CR1-CRE-ER

-CamKCre                     -CB1

-CMVCre                       -D2-Cre

-NestinEGFP

-Fat

-GFAP-Cre-ERT2

-Cx3CR1-GFP

-SRY-MYO (sexage)

 

Equipment: Thermocycler Applied Biosystems, Imager Gel Doc. U-Genius Syngene, PHARE software

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Contact

jean-christophe.helbling@inra.fr

tel:33+557579524

Modification date : 14 August 2023 | Publication date : 26 March 2014 | Redactor : CS